The second edition of the Lifestyle Study on Women’s Health is a timely addition to the literature, highlighting the best evidence on women’s health. This new edition provides new information, an in-depth review of scientific evidence and methodological advances in biomechanics, as well as new research such as integrative omics. These articles highlight changes in the field based on recent lines of research, technological advances, and applications to practice and policy. There are new chapters on endometriosis, lung function, awareness, gynecological cancer, integrated omics, gender discrimination, violence, healthcare and translation knowledge. Each chapter reflects the author’s thoughts on a central life theme to provide a consistent approach throughout the book. The meaning of this topic is summarized in the introductory section, which provides a summary of each topic. The final section summarizes the main findings, common themes, and theoretical and methodological challenges. More than 50 international researchers studying women’s health and well-being from various disciplines contributed to this new edition, which is recommended as essential reading for all lovers of women’s health.