Volume 594 of the Methods in Enzymology series, titled “A Structure-Function Toolbox for Membrane Transporter and Channels,” carries on the esteemed tradition of this renowned serial by featuring high-quality chapters written by experts in the field. This updated release encompasses various aspects of membrane transporters and channels, including Cryo-EM analysis of membrane proteins within nanodics, Solid-Supported membrane-based electrophysiology techniques, Saposin-lipoprotein scaffolds for structure determination, Single-molecule FRET studies on transporters, NMR investigations into the dynamics of channels and transporters, and comprehensive structure-function studies. Additionally, the volume includes a dedicated section on MemStar, a novel GFP-based expression and purification system designed specifically for transporters and channels.

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