Personnel employed in the field of assisted reproductive technology often face a lack of opportunities for specialized training in the specific techniques and technologies required for these procedures. Consequently, the success rates in terms of live birth can vary significantly, ranging from over 50% to less than 10% per treatment cycle. This comprehensive introductory textbook serves as an indispensable resource for trainee embryologists, medical students, and nurses. It thoroughly explores the recent advancements in biotechnology and molecular biology that are integral to the delivery of assisted reproductive services. The book covers fundamental knowledge, including the development and physiology of both male and female reproductive systems, while also delving into practical aspects of in vitro fertilization (IVF), such as gamete and embryo manipulation, cryopreservation, and genetic testing, providing detailed explanations. Furthermore, it offers a comprehensive description of the optimal structure and management of the IVF laboratory, ensuring that procedures are conducted safely and effectively. The inclusion of extensive and highly detailed color illustrations enhances the content, facilitating readers’ comprehension.