This book provides a comprehensive understanding of biomolecular mechanisms through the study of kinetics. With advancements in instrumentation and sample preparation, the field has become more accessible to a broader audience. Many individuals may find traditional textbook explanations too simplistic, while books on chemical kinetics may seem too complex and unrelated to biological samples. By utilizing numerical simulations, this book brings analytical equations to life without requiring advanced mathematical knowledge. In addition to theoretical background, the book also covers practical aspects of conducting kinetic measurements, such as the stopped-flow method and modern continuous-flow techniques. The text explores the benefits and challenges of single-molecule kinetics. Furthermore, computational methods, including simple spreadsheet programs, are discussed as tools for illustrating key concepts and facilitating data analysis.