Despite the increasing success and standardization of assisted reproduction techniques (ART), there is still a limited understanding of what truly constitutes an embryo’s environment. The challenge lies in replicating the complex physiological conditions that the embryo experiences during in-vivo development within the controlled environment of an in-vitro culture. This comprehensive volume provides practical guidance on how to establish and manage an IVF laboratory to create an environment that mimics these conditions, ultimately leading to successful embryo culture. It covers essential aspects such as maintaining air quality and hygiene protocols, optimizing facility layout, and utilizing necessary equipment. The book also delves into topics like consumables, handling techniques, and monitoring systems, while exploring recent advancements in artificial intelligence and automation. This invaluable resource offers a deep understanding of the scientific principles behind embryo culture, which is vital for achieving positive outcomes in ART.