Super-resolution microscopy techniques are covered in this book in both their general and technical details. It discusses the selection criteria for different fluorophores for different SRM techniques and evaluates critically the specific issues that are frequently encountered in real-world applications. In order to achieve the objective of advanced SR imaging studies, a progressive guide for developing the next generation of advanced fluorophores is also presented. Featuring unique content to improve readers’ comprehension of the innovation of more recent SRM technologies, the book is authored by two highly qualified authors. Optical techniques, fluorescent probe design, and algorithm development are a few examples of topics covered in the book. Other topics include: the overall success of SRM in biological inventions; recent highlights and breakthroughs in biology using SRM methods; the future direction and scope of the field; and more. This book is an invaluable resource for chemists and researchers/scientists involved in designing newer fluorescent materials for SRM studies. By guiding them through sample preparation, image processing, and safety measures to be taken in real-world imaging studies, it can also help biologists working on advanced biological studies using SRM.